Investigation the effect of deuterium depleted water and 5-fluorouracil compound on enzymes activity in prostate cancer cell lines

Amir Eshaghiyan¹ Farzaneh Emadi² Mosayeb Eghbali³ Fahimeh Nasimsobhan⁴ Behnaz Nateghi⁵ Farideh Heydari⁶ Nasrin Hadi⁷

1) Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran.
2) Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran.
3) Department of Genetics, Arsanjan Branch, Islamic Azad University, Arsanjan, Shiraz, Iran.
4) Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.
5) Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran.
6) Department of Microbiology, Naein Branch, Islamic Azad University, Naein, Isfahan, Iran.
7) Medical Biotechnology Research Center, Ashkezar Branch, Islamic Azad University, Ashkezar, Yazd, Iran.

Publication : germanconf.com(germanconf.com/2nd)
Abstract :
Prostate cancer is a biologically heterogeneous disease which has become the fifth leading cause of death from cancer in men worldwide. In recent studies, deuterium-depleted water (DDW) therapy has been increasing. Also, studies have shown that 5-Fluorouracil (5-FU) has an anti-cancer effect. Therefore, the aim of this study was to investigate the effect of DDW and 5-FU on the enzyme activity in LN-CAP cell line. The activity of Catalase (CAT) and Malonyl dialdehyde (MDA) enzymes influenced by DDW and 5-FU in different concentrations on the LN-CAP cell line was investigated by the colorimetric method. Statistical analyses were performed with one-way analysis of variance (ANOVA) followed by t-Test. The result of this study showed that in cells treated with the different concentrations of DDW and 5-FU lead to the increase antioxidant activity of the cells.
Keywords : Prostate cancer Deuterium depleted water 5-fluorouracil Enzyme