Agronomic and physiological properties of Nigella sativa as response to humic acid and nano-chelated iron under water deficit

aziz hayati¹ Mohammad Mehdi Rahimi² Mashaallah Hosseini³

1) Department of Agronomy, Yasouj Branch, Islamic Azad University, Yasouj, Iran
2) Department of Agronomy, Yasouj Branch, Islamic Azad University, Yasouj, Iran
3) Associate Professor, Soil and Water Research Department, Fars Agricultural and Natural Resources Research and Education Center, AREEO, Shiraz, Iran

Publication : 7th.International Conference on Researches in Science & Engineering & 4th.International Congress on Civil, Architecture and Urbanism in Asia(7icrsie.com)
Abstract :
Abstract In order to evaluation of humic acid and iron nano-chelated on some physio-chemical properties of Nigella sativa under water stress, an experiment was conducted as split factorial on the basis of randomized complete block design with three replications during two years (2018-2019) in Eghlid Agricultural Research of Fars province. Main plots included three irrigation levels (50 (I1), 75 (I2) and 100% (I3) of available water), sub plots include foliar application of humic acid (0, 250 and 500 mg.L-1) and foliar application of nano-chelate iron (0, 1 and 2g.L-1). Results showed that severe stress (I1) led to reduction of yield (24%), %essential oil (31%), oil percent (3%), RWC (8%), chlorophyll a and b (28%) and increased, catalase (71%), peroxidase (61%), superoxide dismutase (58%), proline (129%) and ABA (77%). Application of humic acid at 250 and 500 (mg.L-1) led to 3 and 16% increase and 1 and 2 (g.L-1) iron nano-chelated showed 90 and 32% increase compare to control. In interactions between irrigation, humic acid and iron nano-chelated under 50 and 75% of available water, the highest means observed in 500 (mg.L-1) humic acid + 2 (g.L-1) iron nano-chelated. In general, humic acid and nano-chelated was suitable for moderate of drought stress.
Keywords : fertilizer peroxidase medical plant ABA catalase