Fabrication of Carbon Paste electrode modified by molecularly imprinted polymers and NiFe2O4 nanoparticles for determination of methocarbamol

Yeganeh Taherkhani¹ Abbas Maleki² Farzaneh Nourifard³ Mahmood Payehghadr⁴

1) Department of Chemistry, Payame Noor University, Alborz , Iran Email:
2) Department of Chemistry, Payame Noor University, Alborz , Iran Email:
3) Department of Chemistry, Payame Noor University, Alborz , Iran Email:
4) Department of Chemistry, Payame Noor University, Alborz , Iran Email:

Publication : 13th Biennial Electrochemistry Seminar of Iran(elect13pguac.ir)
Abstract :
Methocarbamol is used as an adjunct in the short-term symptomatic treatment of painful muscle spasms associated with musculoskeletal conditions and is sometimes given with analgesics for musculoskeletal pain treatment [1]. Methocarbamol is easily absorbed from the intestine and widely distributed in all body tissues. Because of potential for severe side effects, this drug is on the list for high risk medications in the elderly [2,3]. A carbon paste electrode modified with NiFe2O4 nanoparticles and molecularly imprinted polymer (MIP) was developed for determination of methocarbamol. A noncovalent MIP was synthesized using methacrylic acid (MAA) as functional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker and methocarbamol as template, in polymerization solvent and was characterized by furrier transforms infrared (FTIR) spectrometry. The effect of various experimental parameters including percentage of MIP/NiFe2O4 nanoparticle, pH and scan rate on the voltammetric response of methocarbamol was studied. The electrochemical behavior of the NiFe2O4 nanoparticle-MIP carbon paste electrode was investigated by cyclic voltammetry and differential puls voltammety. At the optimum conditions, the concentration of methocarbamol was determined using DPV in a linear range of 7.07 × 10−6 to 2.1 × 10−4 mol L−1 with a correlation coefficient of 0.999. The limit of detection (LOD) was found to be 6.2 × 10−6 mol L−1 and the recovery and relative standard deviation (RSD%) of the method were higher than 97.3% and lower than 3.6%, respectively. The proposed method was successfully applied in the determination of methocarbamol in pharmaceutical and human serum samples.
Keywords : Methocarbamol Imprinted polymer Carbon paste electrode Nanoparticles