Construction of reinforced-scaffold by electrospining of chitosan/nanocrystalline cellulose-graft poly (Nvinylcaprolactam) for tissue engineering

Hessamaddin Sohrabi¹ Parinaz Nezhad-Mokhtari² Marjan Ghorbani³ Mir Reza Majidi⁴

1) Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran
2) Department of Medical Nanotechnology, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran
3) Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4) Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

Publication : International Conference on Emerging Trends in Engineering, Science and Technology(etest2020.com)
Abstract :
In the last years, chitosan (CS) nanofibers as one of the biodegradable biomaterials in nature for tissue engineering and related fields have been widely used. For this purpose, we prepared the reinforced-CS/nanocrystals cellulose-graft-poly(N-vinylcaprolactam) (CS/NCC-g-PVCL) nanofibers via the electrospinning technique. Fabricated nanofibers were characterized and studied for their structural, morphological, as well as mechanical and hydrophilic properties. The uniform nanofibers were achieved, and NCC-g-PVCL contents were partially embedded into CS nanofibers, as revealed in SEM analyses. Incorporation of NCC-g-PVCL contents (0.5, 2.5, and 5 wt %) enhanced the average fiber diameter of the obtained nanofibers from 100 nm (neat CS) to∼350 nm (CS/NCC-g-PVCL (5 wt %)). Additionally, among the CS/NCC-g-PVCL nanocomposite fibers, those loaded with 5 wt % NCC-g-PVCL had the best mechanical properties. Cell culture results presented that the prepared nanofibers had better cytocompatibility and proliferation than neat CS nanofibers.
Keywords : Chitosan Nanocrystals cellulose Poly(N-vinylcaprolactam) Nanofibers Electrospinning tissue engineering.