Evaluation of Herpes Simplex Virus Types 1 and 2 in Patients with Multiple Sclerosis

Aref Atefi¹ Carolin Ebadfardzadeh² Mohamad Tahmasbi³ Gholamreza Shahkamali⁴ Saeid Ferdousmakan⁵ Seyed Mohammad Ghorbani Dazmir⁶ Niloufar Amirebrahimi⁷

1) Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2) Department of Cell and Molecular Biology, Islamic Azad University, Tehran Medical Branch Tehran, Iran
3) Department of Clinical Biochemistry, Medical School, Ahvaz Jondishapur University of Medical Sciences, Ahvaz, Iran
4) Department of Microbiology, Faculty of Science, Kazerun Branch, Islamic Azad University, Shiraz, Iran
5) Department of Pharmacy Practice, Nargund College of Pharmacy, Bangalore 560085,India
6) Faculty of Biological Sciences, Sari Branch, Islamic Azad University, Sari, Iran
7) Faculty member and Head of Microbiology Department, Cancer Biomedical Center (CBC)

Publication : 6th International Conference on Applied Researches in Science & Engineering - Germany(6carse.com)
Abstract :
Herpes simplex virus (HSV) 1 and 2 are probably viral agents responsible for the outbreak of multiple sclerosis (MS). The aim was to determine the demographic characteristics of 50 patients with MS vs 50 healthy individuals in terms of infection with HSV 1&2. Demographic data were collected using a standard questionnaire. The HSV 1&2 infection rate was determined by the loop mediated isothermal amplification method, in which the primers were designed by Primer Explorer V4. Data were analyzed by SPSS version 16 using simple statistical tests. Eleven samples out of 50 patients with multiple sclerosis were positive for HSV 1& 2; however, all tests of the control group were negative (P value= 0.001). From 11 MS patients infected with HSV, 72.7% were female. In those with positive HSV reaction, the average age was 40.36±12.84 years; 9 patients (81.8%) were married; 27.3% had family history of MS; and 54.5% of the patients had relapsing-remitting MS. Performing HSV detection tests is recommended for patients with MS, especially pregnant women, because HSV may cause recurrent miscarriages in women and most HSV infected individuals are female.
Keywords : Demographics; Herpes Simplex Virus; Infection; Multiple Sclerosis