Loop Mediated Isothermal Amplification(LAMP) approaches for detection and identification of Bacillus anthracis

Aref Atefi¹ Zahra Rajabloo² Farzam Fathi³ Carolin Ebadfardzadeh⁴ Seyed Mohammad Ghorbani Dazmiri⁵ Hossein Goodarzi⁶ Parisa Dehghan⁷

1) Hematology and Oncology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2) Department of Microbiology, School of basic Sciences, Sciences and Research Branch, Islamic Azad University, Tehran, Iran
3) Department of Microbiology, Faculty of Science, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
4) Department of Cell and Molecular Biology, Islamic Azad University, Tehran Medical Branch, Tehran, Iran
5) Faculty of Biological Sciences, Sari Branch, Islamic Azad University, Sari, Iran
6) Department of Parasitology, Faculty of Biological Sciences, Azad University Science and Research Branch, Tehran, Iran
7) Department of Inorganic Chemistry , Faculty of Chemistry sciences, Yazd Branch, Islamic Azad University, Yazd, Iran

Publication : 6th International Conference on Applied Researches in Science & Engineering - Germany(6carse.com)
Abstract :
Anthrax is an acute and sub-acute disease caused by Bacillus anthracis which is grouped under the highly dangerous biological agents (category A). Classical procedures for B. anthracis detections involve culture, gamma-phage lysis, immunologic tests that are time consuming and their implementation requires expensive equipment. Loop mediated isothermal amplification (LAMP) is an innovative molecular technique developing as a simple rapid diagnostic tool for detection and identification of microbial diseases in which reagents react rapidly and efficiently, with a high specificity and sensitivity, shorten the detection process, without nucleic acid extraction and without specialized thermal cycling equipment. Moreover, LAMP is cost effective and portable. As a result, this technique can be used as a valuable tool for rapid and cost effective for detection of B. anthracis from different bacteria subfamilies in cases of bioterrorism and can be a valuable tool for fast and efficient detection of this lethal bacteria. This mini-review discusses examined the findings reported on the use of a modern technique of Loop mediated isothermal amplification for the detection of B. anthracis.
Keywords : Bacillus anthracis; Molecular Diagnostic; Loop-mediated Isothermal Amplificatio