Polycaprolactone/ Keratin/ Platelet-rich fibrin nanofiber scaffold as wound dressing: in vitro and in vivo study

Marjan Mirhaj¹ Saeideh Salehi² mohamadreza tavakoli³ Jaleh Varshosaz⁴ Sheyda Labbaf⁵

1) Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
2) Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
3) Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran
4) Department of Pharmaceutics, school of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Iran
5) Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran

IOI Code : XECE-GGAKF
Publication : International Conference on Recent Advances in Engineering, Innovation and Technology - Belgium(eitconf.com/1st)
Abstract :
    Recently, nanofibrous structures have shown great potential for a wide range of medical applications. The aim of the current study was to evaluate the wound healing process using Polycaprolactone/Keratin/Platelet-rich fibrin (PCL/Kr/PRF) fibrous scaffold fabricated through electrospinning process. A range of techniques were utilized to fully characterize the chemical, physical and biological properties of the resultant structure. Results revealed that by the addition of only 0.5%w/v PRF to PCL/Kr (PCL/Kr/0.5PRF) sample, the fibers diameter decreased from 193.93 ± 64.80 nm to 65.98 ± 14.03 nm, and the stress at break demonstrated a 18.27% increase in comparison to the PCL sample (from 2.90 ± 0.80 MPa to 3.43 ± 0.90 MPa). The PCL/Kr/0.5PRF scaffold showed more antibacterial activity against gram-positive and gram-negative bacteria than PCL/Kr sample. Based on enzyme-linked immunosorbent assays, the PCL/Kr/0.5PRF sample revealed an independent release of VEGF and PDGF for 7 days. Cell viability studies demonstrated non-cytotoxic nature of PRF-containing dressings. Also, chorioallantoic membrane (CAM) assay was performed to evaluate the angiogenic potential of the wound dressings. The in vivo assessments also showed that PCL/Kr/0.5PRF accelerated the wound healing process in terms of collagen deposition and the formation of skin appendages which was comparable to the normal skin. Overall, the data presented in this study greatly suggest that the PCL/Kr/0.5PRF wound dressing could be a suitable candidate for wound healing and skin regeneration.

Keywords : keratin platelet rich fibrin fiber wound dressing wound healing