Isolation and characterization of Lipase Producing halophilic Bacteria from salty soils in the central desert of Iran

Fahimeh Mahmoudnia¹

1) Department of Biology, Faculty of Science, Farhangian University, Tehran, Iran.

IOI Code : XEEA-HBAZD
Publication : 4th International Congress On Engineering, Technology and Innovation(eticong.com/4th)
Abstract :
    The desert region in central Iran has salty soils. The average salinity of these soils is 210 grams per 1 cubic meter of soil with an acidity of 6.8. This desert is a place to isolate salt-loving bacteria in order to gain more information about the microbial diversity of the region. To achieve this goal, samples were collected from different places. Then the samples were diluted and cultured on agar medium (molten haloid agar) with different concentrations of salt (5-35%) and incubated in aerobic conditions at 37 degrees Celsius for 7 days. In general, 205 strains were grown in environments containing (5-15%) salt. Among the isolated strains, 18 strains grew in an environment containing 15-35% salt concentration, which can be considered highly salt-loving bacteria. The evaluation of phenotypic and biochemical identification of the isolated microorganisms showed that 18 extreme halophile strains have the ability to produce lipase enzymes and belong to the Halobacteriacea family of the Halobacteria group. According to the results obtained from the present research, it can be seen that there is a great diversity of salt-loving bacteria in these areas, and this area can be a very suitable place for research projects on salt-loving bacteria.

Keywords : salt soils salt loving bacteria enzymes of halophilic bacteria