Aegle marmelos; investigation on its antimicrobial activities
Aegle marmelos; investigation on its antimicrobial activities
Mina Mobram1 Prakash G Williams2 Ananthakrishnan J Nair3
1) 1. Department of Biotechnology, University of Kerala, Thiruvananthapuram, Kerala, India,
2) 2. Department of Botany and Biotechnology, Bishop Moore College, University of Kerala, Kallumala, Kerala, India,
3) 3. Department of Biotechnology, University of Kerala, Thiruvananthapuram, Kerala, India,
محل انتشار :
هشتمین کنفرانس بین المللی پژوهش در علوم و مهندسی و پنجمین کنگره بین المللی عمران، معماری و شهرسازی آسیا(8icrsie.com)
Abstract :
The aim of this study is the investigation of the antimicrobial efficacy of leaves, bark, fruit, and fruit shell of Aegle marmelos against microbial strains of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Aspergillus flavus, and Candida albicans. The antimicrobial efficacy of hexane, chloroform, and methanol solvent extracts of Aegle marmelos was evaluated by agar-well diffusion method against selected pathogenic bacterial and fungal strains. Minimum inhibitory concentration (MIC), Minimum bactericidal concentration (MBC), and Minimum fungicidal concentration (MFC) were determined using the macro broth dilution method. Streptomycin 0.125 mg/ml was used as the positive control for the antibacterial test, and Clotrimazole 0.125 mg/ml as the positive control for the antifungal test. Chloroform and methanol extracts of the bark were found to be most effective extracts, followed by chloroform extract of the fruit. Chloroform and methanol extracts of the bark are highly effective against S. aureus, E. coli, A. flavus, and C. albicans and showed moderate inhibition against P. aeruginosa. Chloroform extract of the fruit has shown the highest antifungal activity against C. albicans. The highest activity against P. aeruginosa was seen in bark hexane extract that showed 15 mm zone of inhibition. The results indicated the antimicrobial efficacy of Aegle marmelos extracts and promote the identification of its bioactive compounds. However, further studies are required to explore in order to develop novel drug candidates.
Keywords :
Aegle marmelos, Antibacterial activity, Antifungal activity, LC-MS/MS.