Biotransducer-conjugated nobel metal nanomaterials as nanobiosensors for pathogenic organisms

Atiyeh nomani¹

1) author

IOI Code : XHZE-HHKFE
Publication : (https://ismcongress.ir/)
Abstract :
    Bacground and Aim: Pathogenic organisms are a threat to public health, so their quick and timely diagnosis increases the quality of treatment and public health. Noble metal nanomaterials (NMNs) due to their unique physicochemical properties, high surface area, diverse shapes, size, and composition-dependent features such as surface plasmon resonance and quantum confinement take part in the designing of biosensing nanoplatforms with high sensitivity and performance. NMNs are conjugated with biotransducers (like the DNA, enzymes, antibodies, aptamers, etc) and generate nanobiosensors that possess signal amplifying ability, high specificity, and low concentration-diagnosis ability of biomarkers. According to the physicochemical features of NMNs, biosensing of approaches of NMNs are including localized surface plasmon resonance (LSPR), fluorescence enhancement/quenching, surface-enhanced Ramman spectroscopy (SERS), chemical activity, etc. This review examines biosensors based on biotransducers conjugated to NMNs for biosensing of pathogenic organisms. Methods: In order to select the articles, the papers of the years 2010-2022 were examined in the Google Scholar, PubMed, and Scopus databases with the keywords of metal nanoparticles, biosensors, transducers, and biomolecules. And information extraction from basic studies has been done. The keywords were selected by the MeSH browser. Results: Gold nanoparticles (AuNPs)-conjugated polyclonal antibodies are effective for rapid and sensitive sensing of Escherichia coli. Silver nanoclusters(AgNCs) and cystine-rich signal probe DNA conjugated with AuNPs as electrochemical DNA biosensors showed enhanced selectivity and proper stability for sensitive determination of Salmonella. Anti-RSV polyclonal antibody conjugated to metallic nanoparticles (Cu, Ag, and Au) as LSPR-based biosensors, especially functionalized copper (Cu) nanoparticles, have high specificity for the diagnosis of Respiratory syncytial virus (RSV). Conclusion: Biotransducer-conjugated NMNs can act as nanobiohybrides platforms for high sensitive detection of pathogenic organisms with high specificity and selectivity.

Keywords : metal nanoparticles biosensors transducers biomolecules