The Effects of Culture Medium derived from Mesenchymal Stem Cells on Cultured Limbal Stem Cells

Ayda Ansary¹ Dariush Hamidi Alamdari²

1) MSc, Sanandaj University, Iran
2) PhD, Sanandaj University, Iran

Publication : 3rd International Conference On Research Science And Technology(3rstconf.com)
Abstract :
In recent decades stem cells have been utilized in many clinical aspects based on their unique characterizations involved self-renewal and proliferation. Cornea disorders are one of criteria which are considered as a target for stem cell therapy. Cornea disorders are resulted by limbal area deficiency. Damaged limbal area or lack of proper function lead to limbal stem cell deficiency (LSCD). In LSCD situation, after natural removing of cornea epithelium cells, there are not any substitution by limbal stem cells. Then cornea epithelium is made thin, even is removed in intense cases. Therefore ophtalmia epithelium immigrates toward cornea to compensate removed epithelium cells on it; however they are not able to differentiate. In a parallel path, moucin secreting cells and blood vessels enter to cornea and make it thick and unclear. Stem cell therapy has known an effective therapeutic strategy, in which limbal stem cells that are cultured in vitro, then graft to cornea with LSCD. In this investigation it is aimed to culture limbal stem cells in vitro, and then optimize it by mesenchymal stem cells supernatant treatment. Mesenchymal stem cells are derived from fat tissue and its cultured supernatant is used to promote limbal stem cells proliferation. It has known that mesenchymal stem cell s supernatant involved various cytokines which make an applied path for appropriate limbal stem cell therapy. In this research donated eye samples were provided and limbal area was separated and transferred on human amniotic membrane and were cultured with mesenchymal stem cell s supernatant. Graphpad prism software was used to analyze data. Immunofluorescence assay results prove that limbal stem cells are cultured properly in vitro. Furthermore, mesenchymal stem cell s supernatant could able to promote limbal stem cell proliferation, in which control culture (with a standard media) compared with culture at present of mesenchymal s supernatant are different significantly.
Keywords : Limbal stem cell Mesenchymal stem cell s supernatant human amniotic membrane