Study of carcinogenic activity of carnosic and rosmarinic acid in cancer cell line of Hep- G2

narges najarpour¹ masoud mashhadi akbar boojar²

1) Iran university of kharazmi tehran
2) Iran university of kharazmi tehran

Publication : International Conference on Science and Engineering(icesconf.com)
Abstract :
Abstract Background: As anti-apoptotic properties of polyphenols have been proved in past years. In this study, carcinogenic activity of carnosic and rosmarinic acid in human liver carcinoma cells (Hep- G2) were studied. Method: In this experimental study, Hep -G2 cells were cultured in DMEM supplemented containing bovine fetal serum and antibiotics. Cells with double dilution were then cultured from 0 to 70 µM for 24 h and viability of cells was determined by MTT method. In order to evaluate activity of caspase3 and 9 enzymes after 24 hours of incubation of the cells with treated materials, cells were centrifuged and cell lysis solution was added. This mixture was centrifuged and then an appropriate substrate was added into each enzyme and after incubation, absorbance of the resulted solution was read at 405 nm using a spectrophotometer device. To measure the level of ceramide, a recombinant acid ceramidase enzyme and naphthalene-2, 3-dialdehyde, which is fluorescent and is connected to sphingosine resulted from acid ceramidase, were added to the cell extract and was ultimately determined by HPLC. Results: Carnosic acid increased cell viability and caused no induction of apoptosis to a dose-dependent species in Hep-G2 cells by reducing ceramide levels and decreasing activity of caspase 3 and caspase 9 enzymes. Rosmarinic acid in concentrations of up to 50 µM decreased cell viability by increasing ceramide levels and activity of caspse-3 and caspase 9 enzymes. Conclusion: Although in most cases, polyphenols have caused no induction of apoptosis and have decreased cell viability percentage. However in some cases, they have affected inversely and have caused cell growth.
Keywords : Keywords: Carnosic acid Rosmarinic acid Ceramide Cell line Hep-G2 Caspse-3 and 9.