Preparation and characterization of PLGA–lipid hybrid nanoparticles for controlled release of N-acetyl cysteine to lung cancer cells

parvin ahmaditabar¹

1) aDepartment of Biomedical Engineering, Yazd Branch, Islamic Azade University, Yazd, Iran

Publication : International Conference on Engineering and Applied Science(iceasconf.com)
Abstract :
The use of liposomal and biodegradable polymeric nanoparticles (NPs) to encapsulate and deliver therapeutic compounds has increased because of clinically demand. In this study, we focused on new hybrid carrier; poly (D,L-lactide-co-glycolide) (PLGA)–lecithin nanoparticles for controlled release of N-acetyl cysteine (NAC) to lung cancer cell line. These core–shell NPs consist of (i) a PLGA hydrophobic core, and (ii) a soybean lecithin mono-layer shell, and were synthesized by a nanoprecipitation combined with self-assembly method. Nanoparticles were characterized in terms of surface morphology, FTIR spectroscopy, size distribution, in vitro drug release by high performance liquid chromatography (HPLC) and differential scanning calorimetry (DSC). The results indicate that the hybrid NPs around 20 hr to release 50% of NAC versus 10 hr for PLGA NPs. To evaluate the nanoparticles cytotoxicity, cell cytotoxicity test was carried out on the Cor L105 human epithelial lung cancer cell line and showed 83% cell viability at NPS. Our data suggest that the PLGA–lipid core–shell NPs may be a useful new controlled release drug delivery system.
Keywords : Keywords: Drug delivery system PLGA Nano-liposome N-acetyl-cysteine Nanoprecipitation Lung cancer cell line