Effect of Carbon : Nitrogen ratio on alpha amylase production by Bacillus licheniformis GMAF438149

Effect of Carbon : Nitrogen ratio on alpha amylase production by Bacillus licheniformis GMAF438149

Fahimeh Mahmoudnia1

1) Department of biology, Faculty of science, Islamic azad university, Arak, Iran.

IOI Code : XYEH-BBZZD
Publication : The 2nd International Conference on Engineering Sciences and Technology(2icoesat.com)
Abstract :
    Bacillus licheniformis GMAF438149 is an isolate which produces extra cellular alpha amylase exhibiting activity at a wide temperature range and was thermostable. The Bacillus licheniformis GMAF438149, however, produces considerable yields of the amylase. Our results show that the amylase production is higher in the presence of potato starch , Amongst sugars, xylose supported maximum amylase production. Of the nitrogen sources tested, meat extract and yeast extract were the best organic sources, while inorganic sources were not found to be effective. The Carbon : Nitrogen ratio found to be the optimum was 2:1.

Keywords : nitrogen sources carbon sources amylase production