Novel noninvasive detection methods for wounds caused by the skin squamous cell carcinoma
Novel noninvasive detection methods for wounds caused by the skin squamous cell carcinoma
Farzaneh NayebHabib1 Soheila S. Kordestani2 Parvin Mansouri3 Mohammad Shirkhoda4 Arvin Honari5 Mahnaz ArabAhmadi6
1) PhD, Medical Engineering Department, Amirkabir University of Technology, Tehran, Iran,
2) PhD, Medical Engineering Department, Amirkabir University of Technology, Tehran, Iran,
3) Skin and Stem Cell Research Center, Tehran University of Medical Sciences,
4) Tehran University of Medical science ,
5) Medical Engineering Department, Amirkabir University of Technology, Tehran, Iran,
6) Department of chemistry, Iran University of science and technology, Tehran, Iran, Email:
Publication :
5th International Conference on Research in Science & Technology(5rstconf.com)
Abstract :
Squamous cell carcinoma (SCC) is an uncontrolled growth of abnormal cells arising in the squamous cells, which compose most of the skin’s upper layers (the epidermis). The damaging effects of UV radiation are DNA destruction. Visible light and UVA radiation are liable to cause DNA destruction indirectly by producing reactive oxygen species such as superoxide anion, singlet oxygen, and hydrogen peroxide via biochemical reactions. Also, on the other side of the coin, it would result in production some protein in the blood as a biomarker.
GPR55 overexpression was measured by quantitative real-time PCR. Presence of hydrogen peroxide in urine of patients’ was indicated via reaction between potassium iodide and hydrogen peroxide.
The expression of GPR55 gene was significantly higher in treatment group compared to the control group. Also, the presence of hydrogen peroxide in patient’s urine was detected.
The results of the study suggest that the overexpression of GPR55 and H2O2 in urine of SCC patients can be utilized as a biomarker for detection of skin SCC.
Keywords :
Skin squamous cell carcinoma; wound; GPR55 expression; H2O2; biomarker