Electrode Surface Using Graphene And Gold Nanoparticles Laccase Immobilization For Hexachlorophene Determination

Hamed Khorami¹ Mahdi Moosazadeh² Matin Khorami³

1) Department of Medicinal Chemistry, Ghadr University Of Kochesfahan, Guilan, Iran
2) Psychology Department Islamic Azad University Of Rasht, Guilan, Iran
3) student

Publication : 4th International Congress on Engineering, Technology and Applied Science - Auckland University of Technology(etas2019.com)
Abstract :
Monitoring of Hexachlorophene compounds in the agriculture, food industry and for environmental and medical applications has become more relevant in recent years. Conventional methods for detection and quantification of these compounds, such as spectrophotometry and chromatography, are time consuming and expensive. However, laccase biosensors represent a fast method for on-line and in situ monitoring of these compounds. We discuss the main transduction principles. We divide the electrochemical principle into amperometric, voltammetric, potentiometric and conductometric sensors. We divide optical transducers into fluorescence and absorption. The amperometric transducer method is the most widely studied and used for laccase biosensors. Optical biosensors present higher sensitivity than the other biosensors. Laccase production is dominated by a few fungus genera: Trametes, Aspergillus, and Ganoderma. We present an overview of laccase biosensors used for the determination of Hexachlorophene compounds in industrial applications. Hexachlorophene, also known as Nabac, is an organochlorine compound that was once widely used as a disinfectant. The compound occurs as a white odorless solid, although commercial samples can be off-white and possess a slightly phenolic odor. A method is described for fabrication of an electrochemical biosensor to detect Hexachlorophene based on adsorption immobilization of Lac onto gold- cetyltrimethylammonium bromide electrodeposited onto a glassy carbon electrode that is modified with graphene oxide physically. The modified electrode was characterized by voltammetry, and Scanning Electron Microscopy (SEM) techniques.
Keywords : Biosensor; laccase; graphene oxide; gold–cetyltrimethylammonium bromide complex; Hexachlorophene